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Qual é a medida do maior ângulo central formado pelos ponteiros a 240 B 120 C 180 D 270?

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Qual é a medida do maior ângulo central formado pelos ponteiros a 240 B 120 C 180 D 270?

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Recombinant Human TGF-beta 1 Protein Summary

Product Specifications

Purity

>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit the IL-4-dependent proliferation of HT‑2 mouse T cells. Tsang, M. et al. (1995) Cytokine 7:389. The ED50 for this effect is 0.04-0.2 ng/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human TGF-beta 1 protein
Ala279-Ser390

N-terminal Sequence
Analysis

Ala279

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

12.8 kDa (monomer)

SDS-PAGE

12 kDa, reducing conditions
24 kDa, non-reducing conditions

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Qual é a medida do maior ângulo central formado pelos ponteiros a 240 B 120 C 180 D 270?

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Cat # 240-GMP

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Cat # ACFP240

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Cat # 206-IL

Qual é a medida do maior ângulo central formado pelos ponteiros a 240 B 120 C 180 D 270?

Cat # 220-BB

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Cat # BAF240

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Cat # 201-LB

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Cat # BT-002

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Cat # 1290-IL

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Cat # 10291-TA

Complete Your Experiment

Qual é a medida do maior ângulo central formado pelos ponteiros a 240 B 120 C 180 D 270?

Cat # 201-LB

Qual é a medida do maior ângulo central formado pelos ponteiros a 240 B 120 C 180 D 270?

Cat # 206-IL

Qual é a medida do maior ângulo central formado pelos ponteiros a 240 B 120 C 180 D 270?

Cat # 1290-IL

Qual é a medida do maior ângulo central formado pelos ponteiros a 240 B 120 C 180 D 270?

Cat # ACFP240

Product Datasheets

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240-B (with carrier)

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240-B/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

240-B

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Purified recombinant human TGF-beta 1 is an extremely hydrophobic protein that adheres strongly to surfaces. To ensure recovery, reconstitute 2 μg vials at 20 μg/mL in sterile 4 mM HCl containing 1 mg/mL human or bovine serum albumin. Reconstitute 10 μg or larger vials at 100 μg/mL in sterile 4 mM HCl containing 1 mg/mL human or bovine serum albumin. 
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

240-B/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute 2 µg vials at 20 µg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin. Reconstitute 10 µg or larger vials at 100 µg/mL in sterile 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Recombinant Human TGF-beta 1 (Catalog # 240-B) inhibits Recombinant Mouse IL-4 (Catalog # 404-ML) induced proliferation in the HT-2 mouse T cell line. The ED50 for this effect is 0.04-0.2 ng/mL.

1 μg/lane of Recombinant Human TGF-beta 1 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing single bands at 12 kDa and 24 kDa, respectively.

Epithelial to Mesenchymal Transition (EMT) was induced in the A549 human lung carcinoma cell line with cell culture media supplemented with Recombinant Human (rh) TGF-beta 1 (Catalog # 240-B). Control cells were cultured without rhTGF-beta 1. EMT induction was confirmed at 48 h by flow cytometric staining for E-Cadherin (filled; Catalog # FAB18381P), an epithelial cell marker, or an isotype control (Catalog # IC0041P). TGF-beta 1 decreased the expression of E-Cadherin.

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Background: TGF-beta 1

TGF-beta 1 (transforming growth factor beta 1) is one of three closely related mammalian members of the large TGF-beta superfamily that share a characteristic cystine knot structure (1‑7). TGF-beta 1, -2 and -3 are highly pleiotropic cytokines that are proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition (1‑4). Each TGF-beta isoform has some non‑redundant functions; for TGF-beta 1, mice with targeted deletion show defects in hematopoiesis and endothelial differentiation, and die of overwhelming inflammation (2). Human TGF‑ beta 1 cDNA encodes a 390 amino acid (aa) precursor that contains a 29 aa signal peptide and a 361 aa proprotein (8). A furin‑like convertase processes the proprotein to generate an N‑terminal 249 aa latency‑associated peptide (LAP) and a C‑terminal 112 aa mature TGF‑ beta 1 (8, 9). Disulfide‑linked homodimers of LAP and TGF‑ beta 1 remain non‑covalently associated after secretion, forming the small latent TGF‑ beta 1 complex (8‑10). Covalent linkage of LAP to one of three latent TGF‑ beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix (9, 10). TGF‑ beta is activated from latency by pathways that include actions of the protease plasmin, matrix metalloproteases, thrombospondin 1 and a subset of integrins (10). Mature human TGF‑ beta 1 shares 100% aa identity with pig, dog and cow TGF‑ beta 1, and 99% aa identity with mouse, rat and horse TGF‑ beta 1. It demonstrates cross‑species activity (1). TGF‑ beta 1 signaling begins with high‑affinity binding to a type II ser/thr kinase receptor termed TGF‑ beta RII. This receptor then phosphorylates and activates a second ser/thr kinase receptor, TGF‑ beta RI (also called activin receptor‑like kinase (ALK) ‑5), or alternatively, ALK‑1. This complex phosphorylates and activates Smad proteins that regulate transcription (3, 11, 12). Contributions of the accessory receptors betaglycan (also known as TGF‑ beta  RIII) and endoglin, or use of Smad‑independent signaling pathways, allow for disparate actions observed in response to TGF‑ beta in different contexts (11).

References

  1. Derynck, R. and K. Miyazono (2008) Cold Spring Harbor Laboratory Press, 29.
  2. Dunker, N. and K. Krieglstein (2000) Eur. J. Biochem. 267:6982.
  3. Wahl, S.M. (2006) Immunol. Rev. 213:213.
  4. Chang, H. et al. (2002) Endocr. Rev. 23:787.
  5. Lin, J.S. et al. (2006) Reproduction 132:179.
  6. Hinck, A.P. et al. (1996) Biochemistry 35:8517.
  7. Mittl, P.R.E. et al. (1996) Protein Sci. 5:1261.
  8. Derynck, R. et al. (1985) Nature 316:701.
  9. Miyazono, K. et al. (1988) J. Biol. Chem. 263:6407.
  10. Oklu, R. and R. Hesketh (2000) Biochem. J. 352:601.
  11. de Caestecker, M. et al. (2004) Cytokine Growth Factor Rev. 15:1.
  12. Zuniga, J.E. et al. (2005) J. Mol. Biol. 354:1052.

Long Name

Transforming Growth Factor beta 1

Entrez Gene IDs

7040 (Human); 21803 (Mouse); 59086 (Rat); 397078 (Porcine); 100033900 (Equine)

Alternate Names

CEDLAP; DPD1; latency-associated peptide; TGF beta; TGF beta1; TGFB; TGFB1; TGF-beta 1 protein; TGFbeta 1; TGF-beta 1; TGFbeta; TGF-beta-1; transforming growth factor beta-1; transforming growth factor, beta 1

Citations for Recombinant Human TGF-beta 1 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

776 Citations: Showing 1 - 10
Filter your results:

  1. The high mobility group protein HMG20A cooperates with the histone reader PHF14 to modulate TGFbeta and Hippo pathways
    Authors: E Gómez-Marí, M Posavec-Ma, L Zarzuela, L Basurto-Ca, JA Guerrero-M, G Arribas, R Yerbes, M Ceballos-C, M Rodríguez-, M Tomé, RV Durán, M Buschbeck, JC Reyes
    Nucleic Acids Research, 2022;50(17):9838-9857.
    Species: Transgenic Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Pseudohypoxic HIF pathway activation dysregulates collagen structure-function in human lung fibrosis
    Authors: CJ Brereton, L Yao, ER Davies, Y Zhou, M Vukmirovic, JA Bell, S Wang, RA Ridley, LSN Dean, OG Andriotis, F Conforti, L Brewitz, S Mohammed, T Wallis, A Tavassoli, RM Ewing, A Alzetani, BG Marshall, SV Fletcher, PJ Thurner, A Fabre, N Kaminski, L Richeldi, A Bhaskar, CJ Schofield, M Loxham, DE Davies, Y Wang, MG Jones
    Elife, 2022;11(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. RORgammat phosphorylation protects against T�cell-mediated inflammation
    Authors: S Ma, SA Patel, Y Abe, N Chen, PR Patel, BS Cho, N Abbasi, S Zeng, B Schnabl, JT Chang, WJM Huang
    Cell Reports, 2022;38(11):110520.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  4. ACPA Alleviates Bleomycin-Induced Pulmonary Fibrosis by Inhibiting TGF-beta-Smad2/3 Signaling-Mediated Lung Fibroblast Activation
    Authors: D Chen, H Tang, H Jiang, L Sun, W Zhao, F Qian
    Frontiers in Pharmacology, 2022;13(0):835979.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Extracellular Matrix Synthesis and Remodeling by Mesenchymal Stromal Cells Is Context-Sensitive
    Authors: J Burk, A Sassmann, C Kasper, A Nimptsch, S Schubert
    International Journal of Molecular Sciences, 2022;23(3):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Stimulation
  6. Delivery of Mesenchymal Stem Cell in Dialdehyde Methylcellulose-Succinyl-Chitosan Hydrogel Promotes Chondrogenesis in a Porcine Model
    Authors: YC Chen, HJ Liao, YM Hsu, YS Shen, CH Chang
    Polymers, 2022;14(7):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Differentiation
  7. Electrospun Biodegradable alpha-Amino Acid-Substituted Poly(organophosphazene) Fiber Mats for Stem Cell Differentiation towards Vascular Smooth Muscle Cells
    Authors: M Wang, S Lin, K Mequanint
    Polymers, 2022;14(8):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  8. Lipid phosphate phosphatase 3 in smooth muscle cells regulates angiotensin II-induced abdominal aortic aneurysm formation
    Authors: PM Van Hoose, L Yang, M Kraemer, M Ubele, AJ Morris, SS Smyth
    Scientific Reports, 2022;12(1):5664.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Defining the Role of Mitochondrial Fission in Corneal Myofibroblast Differentiation
    Authors: KI Jeon, A Kumar, KT Wozniak, K Nehrke, KR Huxlin
    Investigative Ophthalmology & Visual Science, 2022;63(4):2.
    Species: Feline
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Penfluridol targets acid sphingomyelinase to inhibit TNF signaling and is therapeutic against inflammatory autoimmune diseases
    Authors: YH Chen, RH Liu, YZ Cui, A Hettinghou, WY Fu, L Zhang, C Zhang, CJ Liu
    Arthritis Research & Therapy, 2022;24(1):27.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

FAQs

  1. Will Recombinant Human TGF-beta 1 support the maintenance of either human or non-human cultured cells?

    • TGF-beta 1 performs multiple cellular functions, including cell growth, proliferation, differentiation and apoptosis.  It would be necessary to determine the specific conditions for cell growth/proliferation for the cells being cultured.

  2. Can Recombinant Human TGF beta 1 Protein (Catalog # 240-B or 240-B/CF) be reconstituted in PBS or water?

    • Recombinant Human TGF beta 1 Protein (Catalog # 240-B or 240-B/CF) needs to be reconstituted to 100 μg/mL in sterile 4 mM HCl containing 0.1% BSA or 4 mM HCl. This protein is hydrophobic by nature, so it requires an acidic buffer for reconstitution to go completely into solution. Stability testing has been evaluated on the protein with these reconstitution conditions; we can guarantee performance under these reconstitution conditions. When the protein is diluted with the cell culture media or buffers to the working concentration, the concentration of the acid should be buffered or negligible.

  3. Has the half-life of recombinant human TGF beta 1 been determined?

    • While our Recombinant Human TGF-beta 1 Protein has been evaluated for bioactivity, the half-life of a specific protein will depend on experimental conditions, including the cell number, density, and media content. It is up to the end-user to determine the appropriate concentration and timing when adding Recombinant Human TGF-beta 1 Protein to individual experiments. For techniques and methodologies, we recommend reviewing our list of publications under the Citations tab on the product-specific web page to find reported use of our products in similar experimental layouts.

  4. Is the Recombinant Human TGF-beta 1 Protein (Catalog # 240-B) supplied as the active or latent form?

    • Human TGF-beta 1 is latent when it is bound to another protein, the latency-associated peptide (LAP), but it is active when it is not bound to this peptide. Recombinant Human TGF-beta 1 Protein (Catalog # 240-B) contains only the TGF-beta 1 protein (without LAP), thus it is supplied as the active form of TGF-beta 1.

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Reviews for Recombinant Human TGF-beta 1 Protein

Average Rating: 4.7 (Based on 53 Reviews)

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Recombinant Human TGF-beta 1 Protein

By Anonymous on 06/17/2022

Application: In vitro bioactivity in cell culture

Qual é a medida do maior ângulo central formado pelos ponteiros a 240 B 120 C 180 D 270?

Recombinant Human TGF-beta 1 Protein

By Anonymous on 03/08/2022

Application: Epithelial-Mesenchymal Transition in vitro.

Qual é a medida do maior ângulo central formado pelos ponteiros a 240 B 120 C 180 D 270?

Recombinant Human TGF-beta 1 Protein

By Anonymous on 09/29/2021

Application: In vitro bioactivity in cell culture

Recombinant Human TGF-beta 1 Protein

By Anonymous on 05/13/2021

Application: In vitro bioactivity in cell culture
Reason for Rating: Exhibited activation of cells in gene reporter assay with an EC50 of 1ng/ml.

Activation of TGF-ß reporter cell line from BPS Bioscience.

Qual é a medida do maior ângulo central formado pelos ponteiros a 240 B 120 C 180 D 270?

Recombinant Human TGF-beta 1 Protein

By Anonymous on 04/13/2021

Application: In vitro bioactivity in cell culture

Recombinant Human TGF-beta 1 Protein

By Anonymous on 12/22/2020

Application: In vitro bioactivity in cell culture

Recombinant Human TGF-beta 1 Protein

By Anonymous on 12/02/2020

Application: Cell migration/motility

By Anonymous on 11/16/2020

Application: Immunoassay Standard

Recombinant Human TGF-beta 1 Protein

By Anonymous on 10/28/2020

Application: In vitro bioactivity in cell culture

Recombinant Human TGF-beta 1 Protein

By Anonymous on 10/08/2020

Recombinant Human TGF-beta 1 Protein

By Anonymous on 10/07/2020

Application: In vitro bioactivity in cell culture

Recombinant Human TGF-beta 1 Protein

By Anonymous on 07/02/2020

Application: In vitro bioactivity in cell culture

Recombinant Human TGF-beta 1 Protein

By Anonymous on 06/26/2020

Application: In vitro bioactivity in cell culture

Recombinant Human TGF-beta 1 Protein

By Anonymous on 06/06/2020

Application: In vitro bioactivity in cell culture

Recombinant Human TGF-beta 1 Protein

By Anonymous on 05/23/2020

Qual é a medida do maior ângulo central formado pelos ponteiros a 240 B 120 C 180 D 270?

Recombinant Human TGF-beta 1 Protein

By Mozhdeh Sojoodi on 05/16/2020

Application: In vitro bioactivity in cell culture

Qual é a medida do maior ângulo central formado pelos ponteiros a 240 B 120 C 180 D 270?

Recombinant Human TGF-beta 1 Protein

By Anonymous on 04/20/2020

Application: CellProlif
Reason for Rating: Use SMAD reporter to test TGF-beta response in cell culture

Recombinant Human TGF-beta 1 Protein

By Anonymous on 03/06/2020

Application: In vitro bioactivity in cell culture

Recombinant Human TGF-beta 1 Protein

By Anonymous on 01/08/2020

Application: In vitro bioactivity in cell culture

Recombinant Human TGF-beta 1 Protein

By Yanying Fan on 10/09/2019

Application: In vitro bioactivity in cell culture

Recombinant Human TGF-beta 1 Protein

By Anonymous on 09/10/2019

Application: In vitro bioactivity in cell culture

Recombinant Human TGF-beta 1 Protein

By Anonymous on 08/29/2019

Application: Immunoassay Standard

Qual é a medida do maior ângulo central formado pelos ponteiros a 240 B 120 C 180 D 270?

Recombinant Human TGF-beta 1 Protein

By Anonymous on 08/21/2019

Application: In vitro bioactivity in cell culture

By Vaishali Sarode on 02/15/2019

Application: CellProlif
Reason for Rating: great protein

By Anonymous on 01/31/2019

By Anonymous on 12/17/2018

Application: In vitro bioactivity in cell culture

Use SMAD reporter to test TGF-beta response in cell culture.

Qual é a medida do maior ângulo central formado pelos ponteiros a 240 B 120 C 180 D 270?

Recombinant Human TGF-beta 1 Protein

By Anonymous on 11/07/2018

Application: In vitro bioactivity in cell culture

By Anonymous on 10/08/2018

Application: In vitro bioactivity in cell culture

Recombinant Human TGF-beta 1 Protein

By Anonymous on 09/22/2018

Application: In vitro bioactivity in cell culture

Qual é a medida do maior ângulo central formado pelos ponteiros a 240 B 120 C 180 D 270?

Recombinant Human TGF-beta 1 Protein

By Anonymous on 08/20/2018

Recombinant Human TGF-beta 1 Protein

By Anonymous on 08/20/2018

Application: In vitro bioactivity in cell culture
Reason for Rating: Kit was easy to use.

Recombinant Human TGF-beta 1 Protein

By Anonymous on 07/30/2018

Application: In vitro bioactivity in cell culture

Qual é a medida do maior ângulo central formado pelos ponteiros a 240 B 120 C 180 D 270?

By Anonymous on 06/15/2018

Application: Cell Adhesion

By Dong Hu on 04/18/2018

Application: In vitro bioactivity in cell culture
Reason for Rating: This protein shows really robust effect on Mink lung epithelial cells Mv1Lu, including the robust CDKI induction, SKIP2 downregulation, and the activation of TGFbeta1-reporter.

Qual é a medida do maior ângulo central formado pelos ponteiros a 240 B 120 C 180 D 270?

By Anonymous on 03/28/2018

Application: Binding assay/Protein-protein interaction

Recombinant Human TGF-beta 1 Protein

By Anonymous on 01/24/2018

Application: In vitro bioactivity in cell culture
Reason for Rating: Works very well in tissue culture experiments for pSMAD signal induction and for inducing Ca2+ sparks in muscle cultures

Qual é a medida do maior ângulo central formado pelos ponteiros a 240 B 120 C 180 D 270?

Recombinant Human TGF-beta 1 Protein

By Anonymous on 01/05/2018

Application: Immunoassay Standard

This protein was used as the standard in an ELISA with antibodies MAB240 and BAF240.

Recombinant Human TGF-beta 1 Protein

By Anonymous on 12/01/2017

Application: Binding assay/Protein-protein interaction
Reason for Rating: The protein works perfectly

They work awesome

Qual é a medida do maior ângulo central formado pelos ponteiros a 240 B 120 C 180 D 270?

Recombinant Human TGF-beta 1 Protein

By Anonymous on 09/11/2017

Application: In vitro bioactivity in cell culture

Recombinant Human TGF-beta 1 Protein

By Anonymous on 08/30/2017

Application: In vitro bioactivity in cell culture

Recombinant Human TGF-beta 1 Protein

By Anonymous on 05/17/2017

Application: Media additive for protein or antibody production
Reason for Rating: TGFbeta was used to understand EMT process in PANC1 cells and upon treatment we detected classic EMT as anticipated

EMT was induced in PANC1 using TGF beta. EMT markers at both protein and mRNA level were examined using RT PCR and WB. Typical markers of EMT was seen to be altered after TGFbeta treatment in accordance to the desired effect which is induction of EMT

Qual é a medida do maior ângulo central formado pelos ponteiros a 240 B 120 C 180 D 270?

Recombinant Human TGF-beta 1 Protein

By Angelika Schmidt on 05/02/2017

Application: Stem/Immune cell maintenance or differentiation
Reason for Rating: Very good and reproducible performance across experiments, lots, and primary T cell donors.Note: Storage conditions are important and product needs to be used within a specified time.

Product used for differentiation of human FOXP3+ cells (regulatory T cells, Tregs) from naive CD4+ T cells, within different experimental setups.

You may add these papers to the references for this product. We name the product in these papers:

Éliás S, Schmidt A, Kannan V, Andersson J, Tegnér J (2016): TGF-β Affects the Differentiation of Human GM-CSF+ CD4+ T Cells in an Activation- and Sodium-Dependent Manner. Frontiers in Immunology 2016 Dec 23;7:603. doi: 10.3389/fimmu.2016.00603. PMID: 28066414.

Schmidt A, Éliás S, Joshi RN, Tegnér J (2016): In Vitro Differentiation of Human CD4+FOXP3+ Induced Regulatory T Cells (iTregs) from Naïve CD4+ T Cells Using a TGF-β-containing Protocol. Journal of Visualized Experiments 2016 Dec 30;(118). doi: 10.3791/55015. PMID: 28060341.

Schmidt A, Zhang XM, Joshi RN, Iqbal S, Wahlund C, Gabrielsson S, Harris R, Tegnér J (2016): Human macrophages induce CD4+Foxp3+ regulatory T cells via binding and re-release of TGF-β. Immunology & Cell Biology Sep;94(8):747-62. doi: 10.1038/icb.2016.34. PMID: 27075967.

Schmidt A, Eriksson M, Shang MM, Weyd H, Tegnér J (2016): Comparative Analysis of Protocols to Induce Human CD4+Foxp3+ Regulatory T Cells by Combinations of IL‐2, TGF‐beta, Retinoic Acid, Rapamycin and Butyrate. PLoS ONE 11(2), e0148474. doi:10.1371/journal.pone.0148474. PMID: 26886923.

Qual é a medida do maior ângulo central formado pelos ponteiros a 240 B 120 C 180 D 270?

Recombinant Human TGF-beta 1 Protein

By Mahesh Raundhal on 04/14/2017

Application: T cell differentiation
Reason for Rating: Works well in inducing Foxp3 expression in naive mouse CD4 T cells.

Naive mouse T cells were stimulated with anti-CD3e, anti-CD28, IL-2, and human TGF-beta. Four days later, Foxp3 induction was assessed by intracellular flow cytometry.

Qual é a medida do maior ângulo central formado pelos ponteiros a 240 B 120 C 180 D 270?

Recombinant Human TGF-beta 1 Protein

By Anonymous on 02/20/2017

Application: fibroblast-to-myofibroblast differentiation

TGF-beta stimulation of primary murine lung fibroblasts

Recombinant Human TGF-beta 1 Protein

By Anonymous on 08/08/2016

Application: In vitro bioactivity in cell culture
Reason for Rating: consistent high quality protein that retains activity. Reliable.

Recombinant Human TGF-beta 1 Protein

By Oscar Abilez on 07/23/2016

Application: Stem/Immune cell maintenance or differentiation

Qual é a medida do maior ângulo central formado pelos ponteiros a 240 B 120 C 180 D 270?

Recombinant Human TGF-beta 1 Protein

By Anonymous on 07/21/2016

Application: In vitro bioactivity in cell culture
Reason for Rating: Saw morphology changes

Qual é a medida do maior ângulo central formado pelos ponteiros a 240 B 120 C 180 D 270?

Recombinant Human TGF-beta 1 Protein

By Xiaogang Cheng on 07/21/2016

Application: In vitro bioactivity in cell culture
Reason for Rating: worked as published results

Qual é a medida do maior ângulo central formado pelos ponteiros a 240 B 120 C 180 D 270?

Recombinant Human TGF-beta 1 Protein

By Anonymous on 05/12/2016

Recombinant Human TGF-beta 1 Protein

By Anonymous on 05/12/2016

Application: Cell migration/motility
Reason for Rating: Used the product at 5ng/mL to induce EMT in primary human epithelial cells. Worked well at 48 hours post-induction with confirmation of EMT by microscopy as well as western for protein markers.

Recombinant Human TGF-beta 1 Protein

By Anonymous on 05/12/2016

By Anonymous on 01/13/2016

Application: Stem/Immune cell maintenance or differentiation

Recombinant Human TGF-beta 1 Protein

By Anonymous on 01/13/2016

Application: In vitro bioactivity in cell culture

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Qual tecido fundamental é mais comum no corpo da planta qual sua importância?

Qual a diferença entre o conjunto dos números naturais e o conjunto dos números?
Qual a diferença entre o conjunto dos números naturais e o conjunto dos números?

Qual é a doença que pode ser decorrente de hipotireoidismo ou hipertireoidismo o que causa um sintoma observável na região do pescoço?
Qual é a doença que pode ser decorrente de hipotireoidismo ou hipertireoidismo o que causa um sintoma observável na região do pescoço?

Qual proteína é melhor vegetal ou animal?
Qual proteína é melhor vegetal ou animal?

Qual foi o papel do Estado no desenvolvimento industrial brasileiro?
Qual foi o papel do Estado no desenvolvimento industrial brasileiro?

Qual é a diferença entre inibição enzimática competitiva e não competitiva?
Qual é a diferença entre inibição enzimática competitiva e não competitiva?

Qual o tamanho ideal para uma ilha na cozinha?
Qual o tamanho ideal para uma ilha na cozinha?

É a forma que a mão assume para fazer um sinal Segundo o quadro de CM?
É a forma que a mão assume para fazer um sinal Segundo o quadro de CM?

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